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Full-term devel- opment of mice from nuclear transfer of blastomeres was eventually demonstrated in 1987 buy discount valtrex 500mg line hiv infection rates in los angeles. However discount valtrex 1000mg on line antiviral quizlet, the rates were low compared to sheep and cattle, possibly due to differences in the requirements for activation following nuclear transfer. These results emphasize the considerable variation in the success in cloning mammals using blas- tomeres as donor cells. Unlike earlier results using nonmammalian species, serial nuclear transplantation did not offer any substantial improvement in developmen- tal potential. Nonetheless, results from mice, rabbits, and cattle all suggest that reprogramming of cellular fates is dramatically restricted in eight-cell embryos and beyond. The prevailing wisdom was thoroughly shaken by the reports of Dolly—a normal sheep that developed to term following nuclear transplantation of a donor nucleus from a single mammary epithelial cell. Not only was Dolly cloned from somatic cells but it was from adult cells providing a dra- matic confirmation of the earlier work of Gurdon (1970). This was followed by nuclear transplantation of embryonic fibroblasts to clone cattle, sheep, and goats. Cumulus cells from adult animals have also been used as donor cells to clone mice and cattle. The results from animals cloned using somatic cells from mammals substan- tiate much of the work performed in amphibians; however, the data are far from complete (summarized in Table 2. It is clear that a variety of somatic cell types are capable of undergoing nuclear reprogramming following nuclear trans- plantation and yield live offspring. However, efficiency of nuclear reprogram- ming is very dependent on the donor cells. Cumulus cells and fetal fibroblasts have proven to be competent donors in two species, whereas trophectodermal cells were consistently negative in two studies.

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An organization such as a university discount valtrex 500mg overnight delivery boots antiviral foam norovirus, society order valtrex 1000mg with amex antiviral botanicals, association, corporation, or governmental body may serve as an author. Airborne radioactivity survey of the Tabernacle Buttes area, Sublette and Fremont Counties, Wyoming [remote-sensing map]. Rossiiskoe Respiratornoe Obshchestvo [Russian Respiratory Society] Box 7 continues on next page... Tis rule ignores some conventions used in non-English languages to simplify rules for English-language publications. Follow the same rules as used for author names, but end the list of names with a comma and the specifc role, that is, editor or translator. Separate the surname from the given name or initials by a comma; follow initials with a period; separate successive names by a semicolon and a space. Map with no author (cartographer) provided Author Affiliation for Maps (optional) General Rules for Author Affiliation • Enter the afliation of all authors or only the frst author • Begin with the department and name of the institution, followed by city and state/ Canadian province/country • Use commas to separate parts of the address • Place the afliation in parentheses. If you abbreviate a word in one reference in a list of references, abbreviate the same word in all references. Tis rule ignores some conventions used in non-English languages to simplify rules for English-language publications. Moskva becomes Moscow Wien becomes Vienna Italia becomes Italy Espana becomes Spain Box 13. Map showing author (cartographer) afliation Title for Maps (required) General Rules for Title • Enter the title of a map as it appears in the original document, in the original language • Capitalize only the frst word of a title, proper nouns, proper adjectives, acronyms, and initialisms • Use a colon followed by a space to separate a title from a subtitle, unless some other form of punctuation such as a question mark, period, or an exclamation point is already present • Follow non-English titles with a translation whenever possible; place the translation in square brackets • End a title with a space Specific Rules for Title • Area of map not indicated in the title • Titles not in English • Titles in more than one language • Titles containing a Greek letter, chemical formula, or another special character • No title can be found Box 14. Madrid: Comunidad de Madrid, Consejeria de Sanidad, Direccion General Planifcacion Sanitaria; 2000. Beijing Shi ji ben yi liao bao xian ding dian yi liao ji gou ding dian ling shou yao dian zhi nan tu [map]. Tis rule ignores some conventions used in non-English languages to simplify rules for English-language publications.

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